25-5-16S細(xì)胞
數(shù)量： 大量
ATCC Number： HB-37?
細(xì)胞形態(tài)： **樣
**類型： IgM
生長狀態(tài)： 懸浮生長
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
運(yùn)輸方式： 凍存運(yùn)輸
Designations: 25-5-16S
Depositors: DH Sachs, K Ozato
25-5-16S細(xì)胞Isotype: IgM
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against I-A b; cytotoxic
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Comments: 25-5-16S細(xì)胞Animals were immunized with spleen cells from C3H.SW mice.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
No cross-reactions are known.
The antibody reactivity corresponds to the Ia.20 serologic specificity.
Tested and found negative for ectromelia virus (mousepox).
The line was recloned in 1988 at the ATCC from the original deposit.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
25-5-16S細(xì)胞fetal bovine serum to a final concentration of 10%
1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 906: Ozato K, Sachs DH. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J. Immunol. 126: 317-321, 1981.25-5-16S細(xì)胞 PubMed: 6935293
