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產(chǎn)品資料

E.H. IV (Elaine IV)細胞

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產(chǎn)品名稱: E.H. IV (Elaine IV)細胞
產(chǎn)品型號: E.H. IV (Elaine IV)
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無相關(guān)文檔

簡單介紹

E.H. IV (Elaine IV)細胞應(yīng)如何避免細胞污染,細胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因為無菌操作技術(shù)不當(dāng)、操作室環(huán)境不佳、污染之血清和污染之細胞等。嚴格之無菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細胞來源和培養(yǎng)基配制是減低污染之*好方法。E.H. IV (Elaine IV)細胞何時須更換培養(yǎng)基?視細胞生長密度而定,或遵照細胞株基本數(shù)據(jù)上之更換時間,按時更換培養(yǎng)基即可。


E.H. IV (Elaine IV)細胞  的詳細介紹

E.H. IV (Elaine IV)細胞

運輸方式: 凍存運輸

細胞類型: B**細胞

是否是腫瘤細胞: 0

物種來源: 人

生長狀態(tài): 懸浮生長

ATCC Number: CCL-104?

相關(guān)**: 其他**

細胞形態(tài): **樣

數(shù)量: 大量

E.H. IV (Elaine IV)細胞器官來源: 外周血

年限: 20 years

Designations: E.H. IV (Elaine IV)

Depositors: W Henle

Biosafety Level: 2 [Cells Contain HERPESVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast


Source: Organ: peripheral blood

Disease: infectious mononucleosis

Cell Type: E.H. IV (Elaine IV)細胞B lymphoblast;

Cellular Products: Epstein-Barr virus (EBV)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Virus Resistance: partially resistant to poliovirus and vesicular stomatitis viruses

Isoenzymes: G6PD, B

Age: 20 years

Gender: female

Ethnicity: Caucasian

Comments: The cells carry and produce Epstein-Barr virus (EBV).

EBNA positive.

Biological Safety CLASS II.

Propagation:E.H. IV (Elaine IV)細胞 ATCC complete growth medium: RPMI 1640 medium, 80%; fetal bovine serum, 20%

Subculturing: Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days

Cultures can be maintained by addition of fresh medium or by replacement of medium by sedimentation. Establish new cultures by seeding in fresh medium at 5 X 10 exp5 viable cells/ml.

References: 21415: Henle G, et al. Relation of Burkitt's tumor-associated herpes-ytpe virus to infectious mononucleosis. Proc. Natl. Acad. Sci. USA 59: 94-101, 1968. PubMed: 5242134

26274: Niederman JC, et al. Infectious mononucleosis. Clinical manifestations in relation to EB virus antibodies. E.H. IV (Elaine IV)細胞JAMA 203: 205-209, 1968. PubMed: 4864269

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