SBAC細(xì)胞
細(xì)胞形態(tài)： 成纖維樣
運(yùn)輸方式： 凍存運(yùn)輸
組織來源： zona fasciculata; zona reticularis
數(shù)量： 大量
年限： *****
是否是腫瘤細(xì)胞： 0
物種來源： 牛
ATCC Number： CRL-1796?
相關(guān)**： 正常
生長狀態(tài)： 貼壁生長
SBAC細(xì)胞器官來源： 其他
Designations: SBAC
Depositors: MH Simonian
Biosafety Level: 2 [Cells contain Bovine Viral Diarrhea Virus (BVDV) ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Bos taurus
Morphology: fibroblast
Source: Organ: adrenal gland, cortex
Tissue: zona fasciculata; zona reticularis
Disease: normal
Permits/Forms: SBAC細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors: adrenocorticotropic hormone (ACTH)
Age: *****
Comments: The cells are responsive to ACTH and other steroidogenic agents.
SBAC is a normal mammalian endocrine cell type that proliferates in culture, with or without serum, and maintains regulation of its differentiated functions.
Fibroblast growth factor (FGF) stimulates cell division.
The cells have a finite life span in culture (60 to 70 population doublings).
Recent tests for bovine viral diarrhea virus (BVDV) indicate that the line is producing both detectable BVDV antigens and infectious BVDV virions (J. Virol Methods 48:211-221, 1994). [22883]
Propagation: ATCC complete growth medium: Ham's F12 medium with FGF (50 ng/ml of crude FGF - OR - 500 pg/ml of pure FGF), 90%; fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: SBAC細(xì)胞Twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Related Products: recommended serum:ATCC 30-2020
References: 22495: Hornsby PJ, Gill GN. Hormonal control of adrenocortical cell proliferation. Desensitization to ACTH and interaction between ACTH and fibroblast growth factor in bovine adrenocortical cell cultures. J. Clin. Invest. 60: 342-352, 1977. PubMed: 194925
22673: Simonian MH, et al. Clonal growth and culture life span of bovine adrenocortical cells in a serum-free medium. In Vitro Cell. Dev. Biol. 23: 247-252, 1987. PubMed: 3032889
22883: Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438
22976: Simonian MH, et al. Growth and function of cultured bovine adrenocortical cells in a serum-free defined medium. Endocrinology 111: 919-927, 1982. PubMed: 6213403
23510: Gospodarowicz D, et al. Control of bovine adrenal cortical cell proliferation by fibroblast growth factor. Lack of effect of epidermal growth factor. Endocrinology 100: 1080-1089, 1977. SBAC細(xì)胞PubMed: 189990
