RAW 309 Cr.1細胞
細胞類型： 其他細胞類型
是否是腫瘤細胞： 0
物種來源： 小鼠
ATCC Number： TIB-69?
生長狀態(tài)： 貼壁生長
運輸方式： 凍存運輸
數(shù)量： 大量
Designations: RAW 309 Cr.1
Depositors: WC Raschke
RAW 309 Cr.1細胞Biosafety Level: 2
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology:
Source: Cell Type: monocyte/macrophage
Cellular Products: Abelson murine leukemia virus (infectious); lysozyme
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Antigen Expression: H-2d; H-2b
Comments: RAW 309 Cr.1細胞RAW 309 Cr.1 was derived from the same tumor as RAW 309F.1.1 (see ATCC TIB-51).
The cells are negative for surface immunoglobulin (sIg-) Thy-1.2 (Thy-1.2-) and Ia (Ia-).
The cells will pinocytose neutral red and phagocytose latex beads and zymosan.
They will also lyse antibody coated sheep erythrocytes.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
RAW 309 Cr.1細胞Temperature: 37.0°C
Subculturing: Protocol: Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 1207: Raschke WC, et al. RAW 309 Cr.1細胞Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261-267, 1978. PubMed: 212198
