S4B6-1細(xì)胞
ATCC Number： HB-10968?
生長狀態(tài)： 懸浮生長
運(yùn)輸方式： 凍存運(yùn)輸
數(shù)量： 大量
細(xì)胞形態(tài)： **樣
**類型： IgG2a
是否是腫瘤細(xì)胞： 0
物種來源： 大鼠
S4B6-1細(xì)胞Designations: S4B6-1
Depositors: DNAX Research Institute of Molecular and Cellular Biology, Inc.
Isotype: IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: S4B6-1細(xì)胞hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against mouse interleukin 2 (interleukin-2, IL-2)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: This is a supplemental deposit of the S4B6 hybridoma (ATCC HB-8794) that has improved antibody production.
Animals were immunized with supernatant from a mouse helper T cell line (LB2-1, see ATCC CRL-8629).
Spleen cells were fused with Sp2/0 myeloma cells.
The cells secrete immunoglobulin (rat IgG2a) for a short period in culture.
To grow as ascites, inject 2 X 10 exp6 cells/ml into BALB/c mice primed one week earlier with 0.5 ml pristane and given 150R to 450R of gamma irradiation.
S4B6-1細(xì)胞The antibody binds to mouse IL-2 and neutralizes mouse IL-2 activity.
It does not react with rat or human IL-2.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 2 X 10(6) cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: S4B6-1細(xì)胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
currently distributed as:ATCC HB-8794
References: 3863: Mosmann TR. Hybridoma cell lines producing monoclonal antibodies which specifically bind to mouse interleukin-2. US Patent 4,690,893 dated Sep 1 1987
