SA13細胞

數量： 大量

細胞形態(tài)： **樣

**類型： IgG; kappa light chain

ATCC Number： HB-8501?

運輸方式： 凍存運輸

生長狀態(tài)： 懸浮生長

是否是腫瘤細胞： 0

物種來源： 人

Designations: SA13

Depositors: Cetus Corp.

SA13細胞Isotype: IgG; kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: human (B cell); human (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against tetanus toxoid

Permits/Forms: SA13細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11,12

D13S317: 11,12

D16S539: 11,12

D5S818: 12,13

D7S820: 9,10,12

THO1: 8,10,9.3

TPOX: 8,10,11

vWA: 16,17,20

SA13細胞Comments: Peripheral blood B cells from a patient that had been immunized with tetanus toxoid were fused with LTR228 lymphoblastoid cells.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: Culture medium, 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005

recommended serum:ATCC 30-2020

References: 3570: Raubitschek AR, et al.SA13細胞 Human lymphoblastoid cell line and hybridomas derived therefrom. US Patent 4,624,921 dated Nov 25 1986