201-45E9細胞

是否是腫瘤細胞： 0

物種來源： 小鼠

細胞形態(tài)： **樣

**類型： IgG1 and IgG2b

ATCC Number： CRL-2670?

運輸方式： 凍存運輸

生長狀態(tài)： 懸浮生長

數(shù)量： 大量

Designations: 201-45E9

Depositors: National Cancer Institute

201-45E9細胞Isotype: IgG1 and IgG2b

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against a v-src synthetic oncogene peptide

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: 201-45E9細胞Animals were immunized with a synthetic polypeptide with the sequence LMCQCWRKDPEERPTF corresponding to peptides 494 to 509 of the v-src oncogene (formerly 273 to 284).

Spleen cells were fused with Sp2/0 myeloma cells.

In ELISA testing, the antibody cross reacts with the homologous region of FES, v-mos, erb B, v-src, FGR, yes, mil, FMS, abl, ros, lck, and MET. Weak activity was detected for PKS and fps.

Binding was not detected for c-mos, MOS, SRC, ros, rel, FMS, HIR, TRK, sea, and kit. In immunoblot testing, a protein of approximately 40 kd is detected in fetal brain.

The IgG1 reactivity of the antibody with the v-src peptide (494 to 509) was confirmed by ELISA.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: 201-45E9細胞Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.

Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium 201-45E9細胞(without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020