RAG細(xì)胞
品系： BALB/c
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
器官來源： 腎臟
ATCC Number： CCL-142?
數(shù)量： 大量
相關(guān)**： 其他**
細(xì)胞形態(tài)： 其他
運(yùn)輸方式： 凍存運(yùn)輸
生長狀態(tài)： 貼壁生長
Designations: RAG
RAG細(xì)胞Depositors: RJ Klebe, FH Ruddle
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology: amoeboid
Source: Organ: kidney
Strain: BALB/c
Disease: renal adenocarcinoma
Cellular Products: kidney specific esterase-2 (ES-2)
Permits/Forms: RAG細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Resistance: poliovirus 1
Comments: This is a non-reverting 8-azaguanine resistant (HPRT -, HGPRT -) clone of the Renal-2a cell line.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 3 times per week
RAG細(xì)胞Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 1025: Klebe RJ, et al. Mapping of a human genetic regulator element by somatic cell genetic analysis. Proc. Natl. Acad. Sci. USA 66: 1220-1227, 1970. PubMed: 4920091
22239: . . Science 145: 709, 1964.
22275: Klebe RJ, et al. Controlled production of proliferating somatic cell hybrids. J. Cell Biol. 45: 74-82, 1970. PubMed: 4318843
22519: Felluga B, et al. Electron microscope observations on virus particles associated with a transplantable renal adenocarcinoma in BALB-cf-Cd mice. J. Natl. Cancer Inst. 43: 319-333, 1969. RAG細(xì)胞PubMed: 5797837
