UC7-13D5細胞

細胞形態(tài)： **樣

**類型： IgG

ATCC Number： CRL-1989?

數(shù)量： 大量

生長狀態(tài)： 懸浮生長

運輸方式： 凍存運輸

是否是腫瘤細胞： 0

物種來源： 倉鼠

Designations: UC7-13D5

UC7-13D5細胞Depositors: JA Bluestone

Isotype: IgG

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Cricetulus migratorius (B cell); Mus musculus (myeloma) deposited as hamster, Armenian (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against mouse gamma/delta T cell receptor (TCR)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. UC7-13D5細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with the G8 mouse T cell clone (TCR gamma/ delta).

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody reacts with the murine T cell receptor gamma/delta TCR.

The antibody is specific for all murine TCR gamma/delta heterodimers.

UC7-13D5細胞Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: 90% complete growth medium, 10% DMSO.

References: 22362: Bluestone JA, et al. UC7-13D5細胞Repertoire development and ligand specificity of murine TCR gamma delta cells. Immunol. Rev. 120: 5-33, 1991. PubMed: 1650760