219細胞

運輸方式： 凍存運輸

數(shù)量： 大量

是否是腫瘤細胞： 0

物種來源： 小鼠

生長狀態(tài)： 懸浮生長

ATCC Number： HB-9371?

細胞形態(tài)： **樣

**類型： IgG2b

Designations: 219 [Anti PDGF Hybridoma #219]

219細胞Depositors: Amgen, Inc.

Isotype: IgG2b

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; 219細胞monoclonal antibody; against platelet derived growth factor B chain (PDGF B, v-sis form)

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Tumorigenic: Yes

Comments: Animals were immunized with affinity purified PDGF B v-sis.

Spleen cells were fused with Sp2/0-Ag14 myeloma cell.

The antibody reacts with both the A and B chains of PDGF v-sis.

It cross-reacts with the platelet form of PDGF.

Propagation: 219細胞ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; heat-inactivated fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: fetal bovine serum, 90%; DMSO, 10%

References: 41495: Thomason AR, Nicolson MA. 219細胞Methods for purification of platelet-derived growth factor. US Patent 5,175,255 dated Dec 29 1992