2018細(xì)胞

細(xì)胞類型： 其他細(xì)胞類型

年限： fetus

組織來源： stroma

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

生長狀態(tài)： 貼壁生長

器官來源： 肝

數(shù)量： 大量

ATCC Number： CRL-10907?

2018細(xì)胞運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： 成纖維樣

Designations: 2018

Depositors: ImClone Systems Inc.

Biosafety Level: 2 [CELL CONTAIN SV40 VIRAL DNA SEQUENCES ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

Morphology: fibroblast

Source: Organ: liver

Tissue: stroma

Cell Type: 2018細(xì)胞fibroblast immortalized with SV40 large T antigen

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

This material is cited in a U.S. and/or other Patent or Patent Application, and may not be used to infringe on the patent claims. ATCC is required to inform the Patent Depositor of the party to which the material was furnished.

Age: fetus

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 32.0°C

Subculturing: Protocol:

Remove and discard culture medium.

2018細(xì)胞Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 32?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 32?C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended

Medium Renewal: Every two to three days

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

References: 70741: Lemischka IR. 2018細(xì)胞Nucleic acids encoding hencatoporetic stem cell receptor flk-2. US Patent 5,185,438 dated Feb 9 1993

70742: Lemischka IR. Nucleic acids encoding fragments of hematopoietic stem cell receptor flk-2. US Patent 5,270,458 dated Dec 14 1993

70743: Lemischka IR. Nucleic acids encoding hematopoietic stem cells receptors flk-1. US Patent 5,283,354 dated Feb 1 1994

70744: Lemischka IR. Tyrosine kinase receptor flk-2 and fragments thereof. US Patent 5,367,057 dated Nov 22 1994

70745: Lemischka IR. Tyrosine kinase receptor human flk-2-specific antibodies. US Patent 5,548,065 dated Aug 20 1996

70746: Lemischka IR. Nucleic acids encoding soluble human FLK-2 extracellular domain. US Patent 5,621,090 dated Apr 15 1997

70747: Lemischka IR. Antibodies against tyrosine kinase receptor flk-1. US Patent 5,747,651 dated May 5 1998

70748: Lemischka IR. Method for isolating stem cells expressing flk-1 receptors. US Patent 5,912,133 dated Jun 15 1999