203-7D10細(xì)胞

ATCC Number： CRL-2651?

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

細(xì)胞形態(tài)： **樣

**類型： IgG2a; IgG2b

運輸方式： 凍存運輸

數(shù)量： 大量

生長狀態(tài)： 懸浮生長

Designations: 203-7D10

203-7D10細(xì)胞Depositors: National Cancer Institute

Isotype: IgG2a; IgG2b

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension but many cells attach

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against a synthetic v-src oncogene peptide

Permits/Forms: 203-7D10細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with a synthetic polypeptide with the sequence (C)GSSKSKPKDPSQRRHS corresponding to peptides 2 to 17 of the v-src oncogene.

Spleen cells were fused with Sp2/0 myeloma cells.

In immunoblot screening, the antibody detects a 60 kd protein in the src transformed T2 cell line.

A number of additional proteins are also detected in cell lines and embryo tissues including a 50 kd protein in the urine of cancer patients as well as the amniotic fluid of pregnant patients.

The reactivity of the antibody with the src peptide (2-17) was confirmed by ELISA.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: 203-7D10細(xì)胞Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.

Maintain cell density between 2 X 10 exp5 and 2 X 10 exp6 viable cells/ml.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 21967: Niman HL, Lerner RA. 203-7D10細(xì)胞Polypeptide-induced monoclonal receptors to protein ligands. US Patent 5,015,571 dated May 14 1991