2D7F10細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

數(shù)量： 大量

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： **樣

**類型： IgA

2D7F10細(xì)胞ATCC Number： CRL-2025?

Designations: 2D7F10

Depositors: KW Ruby

Isotype: IgA

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: 2D7F10細(xì)胞lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against Leptospira pomona type kennewicki

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Tumorigenic: Yes

Comments: Animals were immunized with.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

2D7F10細(xì)胞The antibody binds to agglutinates L. interogans serovar pomona type kennewicki, and binds to type kennewicki antigen in ELISA.

It can be used to identify type kennewicki in veterinary biologicals.

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 24407: . . 2D7F10細(xì)胞Proceedings of the 94th Annual Meeting of the United States Animal Health Association, Denver, CO, : 290-294, 1990.