R17 208.2細(xì)胞
生長狀態(tài)： 懸浮生長
細(xì)胞形態(tài)： **樣
**類型： IgM , IgM
運(yùn)輸方式： 凍存運(yùn)輸
ATCC Number： TIB-220?
數(shù)量： 大量
是否是腫瘤細(xì)胞： 0
物種來源： 大鼠
R17 208.2細(xì)胞Designations: R17 208.2
Depositors: J Lesley
Isotype: IgM , IgM
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: R17 208.2細(xì)胞hybridoma:lymphoblast B lymphocyte; somatic cell hybrid
Cellular Products: immunoglobulin; monoclonal antibody; against mouse transferrin receptor
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with the mouse erythroleukemia line 745.6.
Spleen cells were fused with S194/5.XXO.BU.1 myeloma cells.
This antibody blocks iron uptake and cell proliferation of most murine hematopoietic cell lines and primary cell cultures.
Tested and found negative for ectromelia virus (mousepox).
Propagation: R17 208.2細(xì)胞ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.02 mM 2-mercaptoethanol and 10% horse serum
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Two to four times weekly
Preservation: Freeze medium: Complete growth medium supplemented with 5% DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 996: Trowbridge IS, et al. R17 208.2細(xì)胞Murine cell surface transferrin receptor: studies with an anti-receptor monoclonal antibody. J. Cell. Physiol. 112: 403-410, 1982. PubMed: 6290505
