YN1/1.7.4細(xì)胞
ATCC Number： CRL-1878?
是否是腫瘤細(xì)胞： 0
物種來源： 褐鼠
數(shù)量： 大量
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞形態(tài)： **樣
**類型： IgG2b
生長狀態(tài)： 懸浮生長
Designations: YN1/1.7.4
Depositors: F Takei
YN1/1.7.4細(xì)胞Isotype: IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Rattus norvegicus (myeloma) deposited as rat (B cell); rat (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against murine ICAM-1 (ICAM 1, intercellular adhesion molecule)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. YN1/1.7.4細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with NS-1 mouse myeloma cells.
Spleen cells were fused with Y3 rat myeloma cells.
The antibody reacts with a 95000 to 100000 dalton antigen (ICAM-1) on activated and proliferating murine lymphocytes.
The antibody partially inhibits Con A stimulation of spleen cells but does not inhibit LPS stimulation.
The antibody strongly inhibits mixed lymphocyte responses (MLR).
The ICAM-1 antigen is absent on most unstimulated thymocytes and spleen cells.
Propagation: YN1/1.7.4細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1776: Takei F. Inhibition of mixed lymphocyte response by a rat monoclonal antibody to a novel murine lymphocyte activation antigen (MALA-2). J. Immunol. 134: 1403-1407, 1985. YN1/1.7.4細(xì)胞PubMed: 2981916
