R1-2細胞

運輸方式： 凍存運輸

數(shù)量： 大量

生長狀態(tài)： 懸浮生長

細胞形態(tài)： **樣

**類型： IgG2b

ATCC Number： HB-227?

是否是腫瘤細胞： 0

物種來源： 大鼠

Designations: R1-2

R1-2細胞Depositors: IL Weissman

Isotype: IgG2b

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against LPAM-1 (lymphocyte Peyer's patch HEV adhesion molecule), VLA-4

R1-2細胞Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with the Peyer's patch HEV binding lymphoma line TK1.

Spleen cells were fused with P3X63Ag8.653 myeloma cells.

The antibody recognizes the alpha subunit of a 160000/130000 dalton cell surface heterodimer expressed on lymphocytes and lymphomas capable of binding to high endothelial venules (HEV) of murine Peyer's patch.

This subunit corresponds to the alpha subunit of the human VLA-4 integrin receptor, and the two are structurally closely related (if not identical).

Propagation: ATCC complete growth medium: R1-2細胞RPMI 1640 medium with 0.05 mM 2-mercaptoethanol and 2 mM L-glutamine, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 1794: Holzmann B, et al. Identification of a murine Peyer's patch-specific lymphocyte homing receptor as an integrin molecule with an alpha chain homologous to human VLA-4alpha. Cell 56: 37-46, 1989. R1-2細胞PubMed: 2463092