2E.6細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 大鼠

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

**類型： IgG1

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： HB-128?

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

Designations: 2E.6

Depositors: JM Davie

2E.6細(xì)胞Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse 2E.6細(xì)胞(myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against mouse IgG3

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: 2E.6細(xì)胞Animals were immunized with mouse myeloma proteins of the IgG1 (MOPC-21), IgG2a (HOPC-1), IgG2b (MPC-11), IgG3 (J606 and GAC-39) and IgM (M104E) subclasses.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with non-essential amino acids, 85%; horse serum, 10%; fetal bovine serum, 5%. If desired, the fetal bovine serum may be omitted

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. 2E.6細(xì)胞Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.