2C4細(xì)胞

細(xì)胞形態(tài)： **樣

**類型： IgG2a

ATCC Number： CRL-1760?

生長狀態(tài)： 懸浮生長

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

Designations: 2C4

2C4細(xì)胞Depositors: KL Knight

Isotype: IgG2a

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: 2C4細(xì)胞immunoglobulin; monoclonal antibody; against rabbit Ia (cytotoxic)

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Tumorigenic: Yes

Comments: Animals were immunized with rabbit spleen cells.

Spleen cells were fused with P3X63Ag8.653 myeloma cells.

2C4細(xì)胞The antibody reacts with an isotypic determinant present on all rabbit Ia molecules, and precipitates polypeptides with molecular weights of 28000, 31000 and 35000 daltons (corresponding to the beta, Ii and alpha chains, respectively).

The antibody is cytotoxic, inhibits mixed lymphocyte reactivity by 80% and inhibits responses to LPS (but not to Con A or PHA).

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: RPMI 1640 medium, 85%; horse serum, 10%; fetal bovine serum, 5%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 1314: Lobel SA, Knight KL. The role of rabbit Ia molecules in immune functions as determined with the use of an anti-Ia monoclonal antibody. Immunology 51: 35-43, 1984. 2C4細(xì)胞PubMed: 6197360