2D-7細(xì)胞

生長狀態(tài)： 懸浮生長

運輸方式： 凍存運輸

細(xì)胞形態(tài)： **樣

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

**類型： IgG1

數(shù)量： 大量

ATCC Number： HB-9667?

Designations: 2D-7

Depositors: Johns Hopkins University

2D-7細(xì)胞Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against human centromere protein B (CENP-B)

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Comments: Animals were immunized with human CENP-B peptide.

Spleen cells were fused with P3X63Ag8.653 myeloma cells.

The antibody reacts with a major epitope on human CENP-B.

The binding of the 2D-7 antibody is at least partially blocked by sera from patients with anticentromere antibodies.

2D-7細(xì)胞Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 22021: Earnshaw W, et al. Cloned human centromere autoantigen. 2D-7細(xì)胞US Patent 5,196,307 dated Mar 23 1993