TSHR-T5-51細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

器官來源： 脾

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

年限： *****

品系： BALB/c

細(xì)胞形態(tài)： **樣

**類型： IgG1 kappa

ATCC Number： CRL-2680?

TSHR-T5-51細(xì)胞生長狀態(tài)： 懸浮生長，少量貼壁

Designations: TSHR-T5-51

Depositors: H Loosfelt, E Milgrom

Isotype: IgG1 kappa

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, with some loosely adherent cells

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Organ: spleen

Strain: BALB/c

Cell Type: TSHR-T5-51細(xì)胞hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against human thyroid stimulating hormone (TSH) receptor alpha subunit, extracellular domain

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Tumorigenic: Yes

Age: *****

Comments: Animals were immunized with recombinant human thyroid stimulating hormone (TSH) receptor expressed in. E. coli.

Spleen cells were fused with Sp2/0 mouse myeloma cells.

The antibody recognizes the N-terminal extracellular domain (amino acids domain 19-246), alpha subunit of the human thyroid stimulating hormone (TSH) receptor (hormone binding unit).

TSHR-T5-51細(xì)胞The TSHR-T5-51 (ATCC CRL-2680), TSHR-R5T-44 (ATCC CRL-2681) and TSHR-T5U-317 (ATCC CRL-2682) antibodies are additive. They recognize different epitopes. TSHR-T5U-317 cross reacts with the rodent TSHR.

The antibodies do not interfere with hormone binding and have no effect on the receptor mediated adenylate cyclase activity.

The antibodies can be used in Western Blot, ELISA, immunocytochemistry, and immunohistochemistry assays of the human TSH receptor.

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 80%; heat-inactivated horse serum, 20%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

References: 56150: Loosfelt H, et al. TSHR-T5-51細(xì)胞Two-subunit structure of the human thyrotropin receptor. Proc. Natl. Acad. Sci. USA 89: 3765-3769, 1992. PubMed: 1570295