TSHR-R5T-44細(xì)胞

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)，少量貼壁

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： CRL-2681?

細(xì)胞形態(tài)： **樣

**類型： IgG1; kappa light chain

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

TSHR-R5T-44細(xì)胞Designations: TSHR-R5T-44

Depositors: H Loosfelt, E Milgrom

Isotype: IgG1; kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension (some adherent cells)

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: TSHR-R5T-44細(xì)胞immunoglobulin; monoclonal antibody; against human thyroid stimulating hormone (TSH) receptor alpha subunit, extracellular domain

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Tumorigenic: Yes

Comments: Animals were immunized with recombinant human thyroid stimulating hormone (TSH) receptor expressed in. E. coli.

Spleen cells were fused with Sp2/0 mouse myeloma cells.

The antibody recognizes the N-terminal extracellular domain (amino acids domain 19-246), alpha subunit of the human thyroid stimulating hormone (TSH) receptor (hormone binding unit).

TSHR-R5T-44細(xì)胞The TSHR-T5-51 (ATCC CRL-2680), TSHR-R5T-44 (ATCC CRL-2681) and TSHR-T5U-317 (ATCC CRL-2682) antibodies are additive. They recognize different epitopes. TSHR-T5U-317 cross reacts with the rodent TSHR.

The antibodies do not interfere with hormone binding and have no effect on the receptor mediated adenylate cyclase activity.

The antibodies can be used in Western Blot, ELISA, immunocytochemistry, and immunohistochemistry assays of the human TSH receptor.

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated horse serum, 10%

Temperature: 37.0°C

Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.

Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

Preservation: TSHR-R5T-44細(xì)胞Culture medium, 45%; heat-inactivated horse serum, 45%; DMSO, 10%.

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

References: 56150: Loosfelt H, et al. Two-subunit structure of the human thyrotropin receptor. Proc. Natl. Acad. Sci. USA 89: 3765-3769, 1992. PubMed: 1570295