Lym-1細(xì)胞

ATCC Number： HB-8612?

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

數(shù)量： 大量

生長狀態(tài)： 懸浮生長

細(xì)胞形態(tài)： **樣

**類型： IgG2a

運(yùn)輸方式： 凍存運(yùn)輸

Designations: Lym-1

Depositors: Northwestern University

Lym-1細(xì)胞Isotype: IgG2a

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Lym-1細(xì)胞Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against normal human B cells and derived malignancies

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Comments: Lym-1細(xì)胞Animals were immunized with preparations of nuclei from Raji cells.

Spleen cells were fused with NS-1 myeloma cells.

The antibody stains the surfaces of normal human B cells and derived tumors, but does not react with the surfaces of solid tumors (some cytoplasmic staining is seen with some tumors.

Propagation: ATCC complete growth medium: RPMI 1640 medium, 85%; fetal bovine serum, 15%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: Culture medium, 95%; DMSO, 5%

References: 3901: Epstein AL.Lym-1細(xì)胞 Murine hybridoma Lym-1 and diagnostic antibody produced thereby. US Patent 4,724,213 dated Feb 9 1988