GP+E-86細(xì)胞

品系： NIH/Swiss

細(xì)胞形態(tài)： 成纖維樣

數(shù)量： 大量

生長狀態(tài)： 貼壁生長

運(yùn)輸方式： 凍存運(yùn)輸

年限： embryo

器官來源： 胚胎

細(xì)胞類型： 成纖維細(xì)胞

ATCC Number： CRL-9642?

是否是腫瘤細(xì)胞： 0

GP+E-86細(xì)胞物種來源： 小鼠

Designations: GP+E-86

Depositors: Trustees of Columbia University

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

Morphology: fibroblast

Source: Organ: embryo

Strain: NIH/Swiss

Cell Type: fibroblast

Permits/Forms: GP+E-86細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host (Roche Transfection Reagents)

Age: embryo

Comments: This line is capable of packaging nucleic acids containing a psi packaging sequence into recombinant ecotropic retrovirus genomes.

It can be used to produce retroviral vectors for delivery of foreign genes into susceptible eukaryotic cells.

The line was established by electroporation of two plasmids into NIH 3T3 cells.

One plasmid contained the gag and pol regions of Moloney murine leukemia virus (Mo-MuLV), the other contained the env region of Mo-MuLV.

Propagation: GP+E-86細(xì)胞ATCC complete growth medium: Dulbecco's modified Eagle's medium with 0.015 mg/ml hypoxanthine, 0.25 mg/ml xanthine, and 0.025 mg/ml mycophenolic acid; 90% calf serum, 10%.

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended

Medium Renewal: Twice per week

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

References: 22050: Bank A, et al. GP+E-86細(xì)胞Retroviral packaging cell lines and process of using same. US Patent 5,278,056 dated Jan 11 1994