GP+envAM-12細胞
細胞形態(tài)： 成纖維樣
數(shù)量： 大量
品系： NIH/Swiss
運輸方式： 凍存運輸
生長狀態(tài)： 貼壁生長
年限： embryo
ATCC Number： CRL-9641?
器官來源： 胚胎
細胞類型： 成纖維細胞
是否是腫瘤細胞： 0
物種來源： 小鼠
GP+envAM-12細胞Designations: GP+envAM-12
Depositors: Trustees of Columbia University
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology: fibroblast
Source: Organ: embryo
Strain: NIH/Swiss
Cell Type: fibroblast
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. GP+envAM-12細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: embryo
Comments: This line is capable of packaging nucleic acids containing a psi packaging sequence into recombinant amphotropic retrovirus genomes.
It can be used to produce retroviral vectors for delivery of foreign genes into susceptible eukaryotic cells.
The line was established by electroporation into NIH 3T3 cells of two plasmids that separately encode the the env region of a murine amphotropic MuLV and the gag, pol and other sequences needed for viral packaging.
Propagation: GP+envAM-12細胞ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; newborn bovine calf serum, 10%. After 1 to 3 days, add 0.015 mg/ml hypoxanthine, 0.25 mg/ml xanthine, 0.025 mg/ml mycophenolic acid and 0.2 mg/ml hygromycin B
Temperature: 37.0°C
Subculturing: Medium Renewal: Twice per week
Remove medium, add fresh 0.10% trypsin in phosphate buffered saline for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
References: 22050: Bank A, et al. Retroviral packaging cell lines and process of using same. US Patent 5,278,056 dated Jan 11 1994
32557: Medin JA, et al. Correction in trans for Fabry disease: expression, secretion, and uptake of alpha-galactosidase A in patient-derived cells driven by a high-titer recombinant retroviral vector. Proc. Natl. Acad. Sci. USA 93: 7917-7922, 1996. GP+envAM-12細胞PubMed: 8755577
