2F.11.15細胞

是否是腫瘤細胞： 0

物種來源： 小鼠

ATCC Number： TIB-194?

生長狀態(tài)： 懸浮生長

細胞形態(tài)： **樣

**類型： IgA

數(shù)量： 大量

運輸方式： 凍存運輸

2F.11.15細胞Designations: 2F.11.15

Depositors: H Nariuchi

Isotype: IgA

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: 2F.11.15細胞immunoglobulin; monoclonal antibody; against 2,4,6-trinitrophenyl (TNP)

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Comments: Animals were immunized with TNP-KLH.

2F.11.15細胞Spleen cells were fused with P3X63Ag8.653 myeloma cells.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. 2F.11.15細胞Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.