LYK-1細(xì)胞

運輸方式： 凍存運輸

生長狀態(tài)： 懸浮生長

是否是腫瘤細(xì)胞： 0

物種來源： 大鼠

ATCC Number： HB-306?

細(xì)胞形態(tài)： **樣

**類型： IgG1

數(shù)量： 大量

Designations: LYK-1

LYK-1細(xì)胞Depositors: Y Yamashita, KS Hathcock

Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products:LYK-1細(xì)胞 immunoglobulin; monoclonal antibody; against a determinant on murine CD44 isoforms expressing variable exon V10.

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Comments: Animals were immunized with a fusion protein corresponding to the products of CD44 variable exons V5 through V10.

Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells.

The antibody is specific for a determinant on murine CD44 isoforms expressing variable exon V10.

Propagation: LYK-1細(xì)胞ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate and supplemented with:0.05 mM 2-mercaptoethanol1% Origen Hybridoma Cloning Factor10% fetal bovine serum

Temperature: 37.0°C

Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.

Maintain cultures at cell concentrations between 1 X 10(5) and 8 X 10(5) viable cells/ml.

Preservation: Culture medium, 95%; DMSO, 5%

References: 38505: Yamashita Y, et al. LYK-1細(xì)胞Tissue-specific and growth-regulated expression of CD44 variable exon determinants in the mouse. Cell. Immunol. 176: 22-33, 1997. PubMed: 9070314