2AB1-IA10細(xì)胞

生長狀態(tài)： 懸浮生長

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

**類型： IgG2b

是否是腫瘤細(xì)胞： 0

物種來源： 大鼠

ATCC Number： HB-8210?

運(yùn)輸方式： 凍存運(yùn)輸

Designations: 2AB1-IA10

2AB1-IA10細(xì)胞Depositors: University of Pittsburgh

Isotype: IgG2b

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: 2AB1-IA10細(xì)胞B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against laminin (basement membrane glycoprotein)

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Comments: Animals were immunized with extracellular matrix proteins from M1536-B3 cells.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

2AB1-IA10細(xì)胞The antibody reacts with laminin and can be used to detect laminin in tissues, and for isolation and quantitation of laminin.

Propagation: ATCC complete growth medium: Alpha minimum essential medium, 90%; heat-inactivated horse serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 3880: Chung AE. 2AB1-IA10細(xì)胞Novel hybridoma cell line producing monoclonal antibodies against laminin and a process for the production thereof. US Patent 4,642,293 dated Feb 10 1987