2B8細(xì)胞
ATCC Number： CRL-12569?
相關(guān)**： **瘤
細(xì)胞形態(tài)： **樣
年限： 7 years
細(xì)胞類型： 其他細(xì)胞類型
是否是腫瘤細(xì)胞： 0
物種來源： 人
數(shù)量： 大量
組織來源： ascites
生長狀態(tài)： 懸浮生長
2B8細(xì)胞運輸方式： 凍存運輸
Designations: 2B8
Depositors: Bayer Corporation
Biosafety Level: 2 [Cells contain EBV particles ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Tissue: ascites
Disease: Burkitt's lymphoma
Cell Type: B lymphocyte; transfected with plasmid pSV2neo
Permits/Forms:2B8細(xì)胞 In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: The 2B8 cell line (ATCC CRL-12569) is a HAT sensitive fusion cell line derived from the P3HR-1 Burkitt's lymphoma cell line (ATCC HTB-62).
Age: 7 years
Ethnicity: Black, African
Comments: The 2B8 cell line (ATCC CRL-12569) is a HAT sensitive fusion cell line derived from the P3HR-1 Burkitt's lymphoma cell line (ATCC HTB-62). P3HR1 cells harbor non-immortalizing EBV. P3HR-1 cells were cloned; treated with methanesulfonic acid ethyl ester (MSE) and plated in medium containing 6-thioguanine (6TG). They were selected for deficiency in hypoxanthine-guanine phosphoribosyl transferase (HGPRT-, HPRT-). One of the clones was transfected with the neomycin resistance expression vector pSV2-neo to obtain the 2B8 cell line.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: 2B8細(xì)胞Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation and subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cell concentration between 1.0 X 10 exp5 and 1.0 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: Derivative: HKB-11, ATCC CRL-12568
Derivative: 20B8, ATCC CRL-12582
Derivative: 1G2, ATCC CRL-13005 (PTA-
References: 70149: Cho MS. 2B8細(xì)胞Human hybrid host cell for mammalian gene expression . US Patent 6,136,599 dated Oct 24 2000
