TIMI.4G.1.3細(xì)胞

細(xì)胞類(lèi)型： T**細(xì)胞

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： **樣

品系： C57BL/6

ATCC Number： TIB-38?

相關(guān)**： **瘤

TIMI.4G.1.3細(xì)胞生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

Designations: TIMI.4G.1.3

Depositors: R Hyman

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus

Morphology: lymphoblast

Source: Disease: lymphoma

Strain: C57BL/6

Cell Type: TIMI.4G.1.3細(xì)胞T lymphocyte;

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Antigen Expression: H-2b; Thy-1.2

Comments: This line was derived from TIMI.4 (ATCC TIB-37).

The cells are resistant to 0.1 mM 6-thioguanine.

Tested and found negative for ectromelia virus (mousepox).

Propagation: TIMI.4G.1.3細(xì)胞ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; horse serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 1129: Hyman R. Studies on surface antigen variants. TIMI.4G.1.3細(xì)胞Isolation of two complementary variants for Thy 1.2. J. Natl. Cancer Inst. 50: 415-422, 1973. PubMed: 4702114