2A3A1H細(xì)胞

細(xì)胞形態(tài)： **樣

**類型： IgG1

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

ATCC Number： HB-8555?

2A3A1H細(xì)胞Designations: 2A3A1H

Depositors: Immunex Corporation

Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products:2A3A1H細(xì)胞 immunoglobulin; monoclonal antibody; against human interleukin 2 (interleukin-2, IL-2) receptor

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Comments: Animals were immunized with phytohemagglutinin activated human peripheral blood (PBL) leukocytes.

Spleen cells were fused with NS-1 myeloma cells.

The antibody binds to IL-2 receptors on human leukocytes and blocks both mitogen induced and antigen induced PBL proliferation.

Propagation: 2A3A1H細(xì)胞ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.025 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 6 X 10 exp5 cells/ml.

Preservation: Culture medium, 95%; DMSO, 5%

References: 3994: March CJ, et al. Hybridoma antibody which binds IL-2 receptor. US Patent 4,845,198 dated Jul 4 1989

3995: March CJ, et al. Interleukin-2 receptor. 2A3A1H細(xì)胞US Patent 4,578,335 dated Mar 25 1986