146-03E04 [146-3E4]細(xì)胞

ATCC Number： CRL-2650?

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

細(xì)胞形態(tài)： **樣

**類型： IgG1; kappa light chain

146-03E04 [146-3E4]細(xì)胞Designations: 146-03E04 [146-3E4]

Depositors: National Cancer Institute

Isotype: IgG1; kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, but many cells attach

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products:146-03E04 [146-3E4]細(xì)胞 immunoglobulin; monoclonal antibody; against a synthetic ras oncogene peptide

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Comments: Animals were immunized with a synthetic polypeptide with the sequence YTLVREIRQHKLRKLNPPDESGPGC corresponding to peptides 157 to 181 of the H-ras oncogene.

Spleen cells were fused with Sp2/0 myeloma cells.

The antibody reacts with the synthetic H-ras oncogene peptide. It reacts with the Harvey (Ha-ras) p21 protein but not the Kirsten (Ki-ras) p21 protein.

Propagation: 146-03E04 [146-3E4]細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.

Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

Preservation: 146-03E04 [146-3E4]細(xì)胞culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 21967: Niman HL, Lerner RA. Polypeptide-induced monoclonal receptors to protein ligands. US Patent 5,015,571 dated May 14 1991

55710: Pumiglia K, et al. Raf-1 N-terminal sequences necessary for Ras-Raf interaction and signal transduction. Mol. Cell. Biol. 15: 398-406, 1995. PubMed: 7799948