G-401 [G401]細胞

年限： 3 months

是否是腫瘤細胞： 0

物種來源： 人

生長狀態(tài)： 貼壁生長

ATCC Number： CRL-1441?

相關(guān)**： 其他**

運輸方式： 凍存運輸

器官來源： 腎臟

細胞形態(tài)： 上皮樣

數(shù)量： 大量

G-401 [G401]細胞Designations: G-401 [G401]

Depositors: PT Peebles

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: kidney

Disease: rhabdoid tumor (formerly classified as Wilms' tumor)

Cellular Products: nephroblast growth factor (NB-GF)

Permits/Forms: G-401 [G401]細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11,13

D13S317: 9,14

D16S539: 12

D5S818: 13

D7S820: 11,14

THO1: 8,9.3

TPOX: 8,11

vWA: 16

G-401 [G401]細胞Cytogenetic Analysis: The cell line has the 46, XY karyotype. There are no apparent marker chromosomes. The rate of polyploidy was 2.8%. The presence of the Y chromosome was confirmed in Q-band observation.

Isoenzymes: G6PD, B

Age: 3 months

Gender: male

Ethnicity: Caucasian

Comments: G-401 was deposited as a cell line derived from a Wilms' tumor. Due to a change in the classification of such tumors, the cell line was examined by Garvin et al. and found to be more appropriately classified as derived from a rhabdoid tumor of the kidney. [49833]

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature:G-401 [G401]細胞 liquid nitrogen vapor temperature

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007

recommended serum:ATCC 30-2020

References: 22955: Weissman BE, et al. Introduction of a normal human chromosome 11 into a Wilms' tumor cell line controls its tumorigenic expression. Science 236: 175-176, 1987. PubMed: 3031816

23338: Burrow CR, Wilson PD. A putative Wilms tumor-secreted growth factor activity required for primary culture of human nephroblasts. Proc. Natl. Acad. Sci. USA 90: 6066-6070, 1993. PubMed: 8392186

33153: Karnieli E, et al. The IGF-1 receptor gene promoter is a molecular target for the Ewing's Sarcoma=Wilms' Tumor 1 fusion protein. J. Biol. Chem. 271: 19304-19309, 1996. PubMed: 8702614

49833: Garvin AJ, et al. The G401 cell line, utilized for studies of chromosomal changes in Wilms' tumor, is derived from a rhabdoid tumor of the kidney. Am. J. Pathol. 142: 375-380, 1993. G-401 [G401]細胞PubMed: 8382007