TF-1a細胞

ATCC Number： CRL-2451?

相關**： 白血病

生長狀態(tài)： 懸浮生長

細胞形態(tài)： **樣

年限： 35 years

數(shù)量： 大量

運輸方式： 凍存運輸

器官來源： 骨髓

細胞類型： 其他細胞類型

是否是腫瘤細胞： 0

物種來源： 人

TF-1a細胞Designations: TF-1a

Depositors: X Hu

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens deposited as human

Morphology: lymphoblast

Source: Organ: bone marrow

Disease: erythroleukemia

Cell Type: erythroblast

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Tumorigenic: Yes

Antigen Expression: CD34+, CD38- [51559]

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 13

D13S317: 8,9

D16S539: 9,12

D5S818: 13

D7S820: 12

THO1: 7,9

TPOX: 8

vWA: 15,17

Age: 35 years

Gender: male

Ethnicity: Japanese

TF-1a細胞Comments: TF-1a is a factor-independent variant isolated from the factor-dependent TF-1 cell line (see CRL-2003). [51559]

The cells retain the ability to respond to a variety of cytokines, with a different response pattern from the parental cell line. [51559]

TF-1a, but not TF-1 cells, form colonies in soft agar culture in the absence of any added growth factors, and generate invasive tumors in nude mice. [51559]

There is a slight constitutive activation of the MAP kinase and MEK proteins in TF-1a but not in TF-1 cells. Phenotypically, TF-1 cells are CD34 positive and CD38 positive, whereas TF-1a cells are CD34 positive and CD38 negative. [51559]

TF1-a cells, but not TF-1 cells, are resistant to tumor necrosis factor alpha (TNF-alpha) induced apoptosis.

TF-1a is a model for studying human primitive myeloid progenitor cells and for studying the process of progressive malignant transformation of myeloid cells.

It can be used to study signal pathways involved in the spontaneous and factor-induced growth of the cells.

A culture submitted to the ATCC in April of 1999 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline.

The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 10 exp5 viable cells/ml.

TF-1a細胞Maintain cell density between 3 X 10 exp5 and 3 X 10 exp6 viable cells/ml.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

parental cell line:ATCC CRL-2003

References: 51559: Hu X, et al. Characterization of a unique factor-independent variant derived from human factor-dependent TF-1 cells: a transformed event. Leuk. Res. 22: 817-826, 1998. PubMed: 9716013