131-94H4細(xì)胞

器官來(lái)源： 脾

細(xì)胞形態(tài)： **樣

**類型： IgG1 kappa

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

年限： *****

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

品系： 129GIX+

131-94H4細(xì)胞ATCC Number： CRL-2739?

Designations: 131-94H4

Depositors: National Cancer Institute

Isotype: IgG1 kappa

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Strain: 129GIX+

Organ: spleen

Cell Type: hybridoma: 131-94H4細(xì)胞B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against a synthetic v-myb oncogene peptide

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Age: *****

Gender: female

Comments: Animals were immunized with a synthetic polypeptide with the sequence RRKVEQEGYPQESSKAG(C) corresponding to peptides 119 to 135 of the MYB oncogene formerly v-myb (residues 2 to 18). Spleen cells were fused with Sp2/0 myeloma cells. Using immunoblot techniques, the antibody recognizes a 25 kd protein in BM2 cells and a protein of approximately 100 kd in Molt 4 cells. Protein is also detected in selected embryo tissues.The IgG1 and IgG2b reactivities of the antibody with the v-myb peptide (119 to 135) were confirmed by ELISA. IgG1 was strongly positive while IgG2b was only slightly reactive.

Propagation: 131-94H4細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml.

Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Preservation: Freeze medium:131-94H4細(xì)胞 Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase