130C3/2B/8細(xì)胞

生長狀態(tài)： 懸浮生長

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

細(xì)胞形態(tài)： **樣

**類型： IgG1

ATCC Number： HB-8088?

130C3/2B/8細(xì)胞運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

Designations: 130C3/2B/8

Depositors: Johns Hopkins University

Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

130C3/2B/8細(xì)胞Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against surface (cercariae) glycoprotein on Schistosoma mansoni

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Comments: 130C3/2B/8細(xì)胞Animals were immunized with cercariae glycoprotein from Schistosoma mansoni.

Spleen cells were fused with P3X63Ag8 myeloma cells.

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 0.2 units/ml insulin, 3 mM L-glutamine, 1 mM sodium pyruvate, 0.15 mg/ml oxaloacetic acid and 0.01 M HEPES, 80%; NCTC 135 medium, 10%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: culture medium, 40%; fetal bovine serum, 50%; DMSO, 10%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X

References: 3511: Strand M. Diagnosis and treatment of fluke infections with monoclonal antibodies. 130C3/2B/8細(xì)胞US Patent 4,416,866 dated Nov 22 1983