15F3-1細(xì)胞
ATCC Number： HB-47?
細(xì)胞形態(tài)： **樣
**類型： IgG1
運(yùn)輸方式： 凍存運(yùn)輸
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
數(shù)量： 大量
生長狀態(tài)： 懸浮生長
15F3-1細(xì)胞Designations: 15F3-1
Depositors: MK Gentry
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: 15F3-1細(xì)胞Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against dengue virus type 1 (denguevirus 1)
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Tumorigenic: Yes
Comments: Animals were immunized with dengue virus type 1 antigen prepared from infected mouse brains (the Hawaiian strain was used).
The antibody is type specific.
Spleen cells were fused with P3X63Ag8 myeloma cells.
Antibodies should be prepared as ascites in pristane primed BALB/c mice.
Tested and found negative for ectromelia virus (mousepox).
Propagation: 15F3-1細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 10%
1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 922: Henchal EA, et al. Dengue virus-specific and Flavivirus group determinants identified with monoclonal antibodies by indirect immunofluorescence. Am. J. Trop. Med. Hyg. 31: 830-836, 1982. 15F3-1細(xì)胞PubMed: 6285749
