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產(chǎn)品資料

Hs 892.T Hs細(xì)胞

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產(chǎn)品名稱: Hs 892.T Hs細(xì)胞
產(chǎn)品型號(hào): Hs 892.T Hs
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無相關(guān)文檔

簡(jiǎn)單介紹

Hs 892.T Hs細(xì)胞應(yīng)如何避免細(xì)胞污染,細(xì)胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因?yàn)闊o菌操作技術(shù)不當(dāng)、操作室環(huán)境不佳、污染之血清和污染之細(xì)胞等。嚴(yán)格之無菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細(xì)胞來源和培養(yǎng)基配制是減低污染之*好方法。Hs 892.T Hs細(xì)胞何時(shí)須更換培養(yǎng)基?視細(xì)胞生長密度而定,或遵照細(xì)胞株基本數(shù)據(jù)上之更換時(shí)間,按時(shí)更換培養(yǎng)基即可。


Hs 892.T Hs細(xì)胞  的詳細(xì)介紹

Hs 892.T Hs細(xì)胞

運(yùn)輸方式: 凍存運(yùn)輸

是否是腫瘤細(xì)胞: 1

物種來源: 人

器官來源: 皮膚

年限: 57 years

數(shù)量: 大量

ATCC Number: CRL-7630?

Hs 892.T Hs細(xì)胞相關(guān)**: 其他**

細(xì)胞形態(tài): 混合型

生長狀態(tài): 貼壁生長

Designations: Hs 892.T

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: mixed


Source: Organ: skin

Disease: Hs 892.T Hs細(xì)胞keratoacanthoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Cytogenetic Analysis: modal number = 46; range = 30 to 92

Age: 57 years

Gender: female

Ethnicity: Caucasian

Comments: Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. Hs 892.T Hs細(xì)胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard culture medium.

Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

Hs 892.T Hs細(xì)胞Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting

Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37C.

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