Selection marker in E. coli: Ampicillin-resistance
Selection marker in mammalian cells: None. For transient transfection only
Promoter for in vivo expression in mammalian cells: CMV promoter
Promoter for in vitro cell free system: T7 (for pCMV6-XL4 and pCMV6-XL5) and SP6 for (pCMV6-XL6)
Cloning sites: EcoRI and SalI. While EcoRI is still preserved, SalI is destroyed upon cloning.
Restriction sites for removing insert: NotI. *Two NotI sites are flanking the cloning sites in the vector.
Cell line suitable for transfection: COS, 293, Hela, CHO, NIH3T3, Mouse L cell, etc.
Transcription termination and polyadenylation signals: from human growth hormone (hGH) gene.