pTK-Green Renilla Luc 載體說明 The Thermo Scientific pCMV-Green Renilla Luc vector is a derivative of pMCS-Green Renilla Luc. It contains the luciferase gene under the control of the Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, creating a constitutive expression vector that can be used as a normalization control to account for experimental variation in combination with other reporters. pTK-Green Renilla Luc 載體特征: Weak (TK) constitutive promoter for co-transfection and normalization
 Intracellular Green Renilla luciferase gene, optimized for high expression in mammalian systems
 Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
 Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
 High-copy pUC bacterial DNA replication origin