pCTAP-Shuttle-A限制性內(nèi)切酶位點(diǎn)
pCTAP Shuttle A, 7708 bp version 016001
Enzymes with 1-10 cleavage sites:
#sites -- Bp position of recognition site --
AarI 1 3151
AatII 4 459, 512, 595, 781
Acc65I 1 940
AccI 3 946, 2788, 3726
AccIII 3 1134, 3881, 4108
AclI 1 6087
AcuI 6 1764, 1961, 3367, 5496, 6703
7135
AflIII 4 143, 980, 3921, 4969
AhdI 2 2087, 5857
Alw44I 2 2471, 5283
AlwNI 5 1767, 1925, 2010, 5380, 6197
ApaI 2 2581, 2887
ApoI 7 242, 1157, 1406, 2482, 3754
7243, 7254
AseI 3 4251, 6033, 7693
AvaI 4 959, 1902, 2082, 4186
AvaII 7 2086, 2110, 3135, 3276, 3354
6000, 7094
BamHI 1 4786
BanII 10 1107, 1130, 1603, 1660, 1816
2581, 2887, 3606, 6939, 7512
BbeI 3 3480, 6329, 6577
BbsI 2 1007, 3381
BciVI 3 2464, 5178, 6786
BclI 2 1249, 6419
BfaI 10 930, 1240, 1441, 4297, 5464
5717, 6052, 6137, 6279, 7592
BfrBI 3 2377, 2555, 4780
BglI 7 422, 544, 615, 1045, 1958
4036, 5976
BglII 1 934
BlnI 1 4296
Bme1580I 7 1210, 2471, 2581, 2887, 5283
6520, 6613
BmgBI 3 65, 2131, 4701
BmrI 5 632, 5907, 6202, 6283, 6517
BpmI 5 1059, 1080, 1663, 4320, 5947
Bpu10I 4 965, 1813, 2857, 2953
BpuEI 6 3345, 3735, 5075, 5337, 5614
6969
BsaAI 6 676, 1032, 2361, 6149, 6880
7439
BsaBI 4 1250, 2023, 4148, 6433
BsaHI 8 459, 512, 595, 781, 2527
3480, 6329, 6577
BsaI 5 1718, 2094, 3082, 3582, 5929
BsaWI 9 1134, 1233, 2467, 3881, 4108
5175, 5322, 6311, 6609
BseMII 9 1093, 1172, 1814, 1923, 2858
2954, 5244, 5653, 5819
BseRI 3 3183, 3475, 4095
BseYI 9 1127, 2259, 2346, 2413, 2838
3035, 5273, 6324, 7082
BsgI 4 1833, 2443, 3498, 4454
BsiEI 7 953, 987, 2099, 4174, 4882
5306, 6484
BsiHKAI 9 1083, 1573, 1603, 2471, 3231
3606, 5283, 6687, 6877
BsmBI 3 1169, 2178, 2525
BsmFI 8 499, 650, 818, 2085, 2983
3136, 3720, 6715
BsmI 4 1337, 1436, 2557, 3588
BspCNI 9 1093, 1172, 1814, 1923, 2858
2954, 5244, 5653, 5819
BspHI 1 5689
BspMI 8 1948, 2792, 2850, 3152, 3871
6245, 6474, 6837
BsrBI 5 956, 4317, 4900, 7189, 7583
BsrDI 5 996, 2576, 5916, 6098, 6804
BsrGI 1 192
BssHII 5 2068, 2070, 3326, 3457, 7642
BssSI 4 3487, 3594, 5142, 7170
Bst1107I 1 3726
BstAPI 2 1826, 3045
BstEII 2 3148, 4064
BstXI 1 2565
Bsu36I 1 1092
BtgI 8 698, 1631, 1786, 2585, 3264
3416, 3440, 7010
BtgZI 7 687, 1857, 2801, 6864, 7015
7208, 7427
BtsI 5 1432, 1830, 2201, 3495, 6256
ClaI 1 974
Csp6I 10 193, 556, 581, 636, 669
720, 877, 941, 3216, 6883
DraI 6 1292, 1989, 3747, 3971, 5726
5745
DraIII 2 1032, 7436
DrdI 4 3414, 5071, 6599, 7390
EagI 2 953, 6484
EarI 3 4847, 6927, 7137
Eco47III 3 2256, 3371, 4336
EcoICRI 2 1603, 3606
EcoO109I 5 1042, 2109, 2312, 3275, 3569
EcoRI 1 3754
EcoRV 1 1221
FspI 2 6082, 6678
HgaI 9 855, 949, 982, 1645, 2527
3394, 5071, 5649, 7668
HincII 3 946, 1353, 1867
HindIII 1 968
HpaI 1 1353
KasI 3 3480, 6329, 6577
KpnI 1 940
MluI 1 980
MmeI 5 1738, 1933, 5159, 5343, 7393
MscI 3 268, 3332, 6658
MslI 5 699, 2566, 2631, 3852, 7011
MunI 2 1342, 1886
NaeI 4 1050, 4041, 7078, 7542
NarI 3 3480, 6329, 6577
NcoI 4 698, 1631, 3416, 7010
NdeI 2 571, 4791
NgoMIV 4 1050, 4041, 7078, 7542
NotI 1 952
NsiI 3 2377, 2555, 4780
NspI 9 143, 1623, 2979, 2994, 3099
3921, 4778, 4969, 6979
PacI 2 4771, 7701
PciI 3 143, 3921, 4969
PfoI 2 2126, 2642
PmeI 1 3746
PmlI 1 1032
PpuMI 2 2109, 3275
PsiI 3 1373, 4013, 7311
PspOMI 2 2581, 2887
PstI 5 1746, 1773, 2203, 2936, 6103
PvuI 1 987
PvuII 7 1065, 1928, 2836, 2863, 2939
6322, 6682
RsaI 10 193, 556, 581, 636, 669
720, 877, 941, 3216, 6883
RsrII 1 7093
SacI 2 1603, 3606
SacII 2 1786, 3440
SalI 1 946
SapI 3 4846, 6927, 7137
SexAI 1 3631
SfcI 8 1746, 1773, 2203, 2936, 5234
5425, 6103, 7659
SfoI 3 3480, 6329, 6577
SgrAI 1 186
SmaI 3 1902, 2082, 4186
SmlI 7 959, 3345, 3735, 5075, 5337
5614, 6969
SnaBI 1 676
SphI 3 1623, 3099, 6979
StyI 9 698, 1631, 2316, 3416, 3452
3470, 4296, 6387, 7010
TatI 5 192, 555, 635, 668, 719
TfiI 7 1892, 2433, 3541, 4148, 4944
7063, 7197
TspGWI 4 154, 3715, 4026, 4588
Tth111I 2 1720, 6693
Van91I 3 1012, 1031, 2127
XbaI 1 1239
XcmI 3 2009, 3600, 6387
XhoI 1 959
XmaI 3 1902, 2082, 4186
XmnI 1 7691
ZraI 4 459, 512, 595, 781
Enzymes that do NOT cut molecule:
AflII AgeI AleI AscI AsiSI
BbvCI BlpI BmtI BsiWI BstBI
EcoNI FseI FspAI NheI NruI
PshAI SanDI SbfI ScaI SfiI
SpeI SrfI SspI StuI SwaI