pCTAP-Shuttle-B載體限制性內(nèi)切酶位點(diǎn)
pCTAP Shuttle B, 7709 bp version 016001
Enzymes with 1-10 cleavage sites:
#sites -- Bp position of recognition site --
AarI 1 3152
AatII 4 459, 512, 595, 781
Acc65I 1 940
AccI 3 946, 2789, 3727
AccIII 3 1135, 3882, 4109
AclI 1 6088
AcuI 6 1765, 1962, 3368, 5497, 6704
7136
AflIII 4 143, 980, 3922, 4970
AhdI 2 2088, 5858
Alw44I 2 2472, 5284
AlwNI 5 1768, 1926, 2011, 5381, 6198
ApaI 2 2582, 2888
ApoI 7 242, 1158, 1407, 2483, 3755
7244, 7255
AseI 3 4252, 6034, 7694
AvaI 4 959, 1903, 2083, 4187
AvaII 7 2087, 2111, 3136, 3277, 3355
6001, 7095
BamHI 1 4787
BanII 10 1108, 1131, 1604, 1661, 1817
2582, 2888, 3607, 6940, 7513
BbeI 3 3481, 6330, 6578
BbsI 2 1008, 3382
BciVI 3 2465, 5179, 6787
BclI 2 1250, 6420
BfaI 10 930, 1241, 1442, 4298, 5465
5718, 6053, 6138, 6280, 7593
BfrBI 3 2378, 2556, 4781
BglI 7 422, 544, 615, 1046, 1959
4037, 5977
BglII 1 934
BlnI 1 4297
Bme1580I 7 1211, 2472, 2582, 2888, 5284
6521, 6614
BmgBI 3 65, 2132, 4702
BmrI 5 632, 5908, 6203, 6284, 6518
BpmI 5 1060, 1081, 1664, 4321, 5948
Bpu10I 4 965, 1814, 2858, 2954
BpuEI 6 3346, 3736, 5076, 5338, 5615
6970
BsaAI 6 676, 1033, 2362, 6150, 6881
7440
BsaBI 4 1251, 2024, 4149, 6434
BsaHI 8 459, 512, 595, 781, 2528
3481, 6330, 6578
BsaI 5 1719, 2095, 3083, 3583, 5930
BsaWI 9 1135, 1234, 2468, 3882, 4109
5176, 5323, 6312, 6610
BseMII 9 1094, 1173, 1815, 1924, 2859
2955, 5245, 5654, 5820
BseRI 3 3184, 3476, 4096
BseYI 9 1128, 2260, 2347, 2414, 2839
3036, 5274, 6325, 7083
BsgI 4 1834, 2444, 3499, 4455
BsiEI 7 953, 987, 2100, 4175, 4883
5307, 6485
BsiHKAI 9 1084, 1574, 1604, 2472, 3232
3607, 5284, 6688, 6878
BsmBI 3 1170, 2179, 2526
BsmFI 8 499, 650, 818, 2086, 2984
3137, 3721, 6716
BsmI 4 1338, 1437, 2558, 3589
BspCNI 9 1094, 1173, 1815, 1924, 2859
2955, 5245, 5654, 5820
BspHI 1 5690
BspMI 8 1949, 2793, 2851, 3153, 3872
6246, 6475, 6838
BsrBI 5 956, 4318, 4901, 7190, 7584
BsrDI 5 997, 2577, 5917, 6099, 6805
BsrGI 1 192
BssHII 5 2069, 2071, 3327, 3458, 7643
BssSI 4 3488, 3595, 5143, 7171
Bst1107I 1 3727
BstAPI 2 1827, 3046
BstEII 2 3149, 4065
BstXI 1 2566
Bsu36I 1 1093
BtgI 8 698, 1632, 1787, 2586, 3265
3417, 3441, 7011
BtgZI 7 687, 1858, 2802, 6865, 7016
7209, 7428
BtsI 5 1433, 1831, 2202, 3496, 6257
ClaI 1 974
Csp6I 10 193, 556, 581, 636, 669
720, 877, 941, 3217, 6884
DraI 6 1293, 1990, 3748, 3972, 5727
5746
DraIII 2 1033, 7437
DrdI 4 3415, 5072, 6600, 7391
EagI 2 953, 6485
EarI 3 4848, 6928, 7138
Eco47III 3 2257, 3372, 4337
EcoICRI 2 1604, 3607
EcoO109I 5 1043, 2110, 2313, 3276, 3570
EcoRI 1 3755
EcoRV 1 1222
FspI 2 6083, 6679
HgaI 9 855, 949, 982, 1646, 2528
3395, 5072, 5650, 7669
HincII 3 946, 1354, 1868
HindIII 1 968
HpaI 1 1354
KasI 3 3481, 6330, 6578
KpnI 1 940
MluI 1 980
MmeI 5 1739, 1934, 5160, 5344, 7394
MscI 3 268, 3333, 6659
MslI 5 699, 2567, 2632, 3853, 7012
MunI 2 1343, 1887
NaeI 4 1051, 4042, 7079, 7543
NarI 3 3481, 6330, 6578
NcoI 4 698, 1632, 3417, 7011
NdeI 2 571, 4792
NgoMIV 4 1051, 4042, 7079, 7543
NotI 1 952
NsiI 3 2378, 2556, 4781
NspI 9 143, 1624, 2980, 2995, 3100
3922, 4779, 4970, 6980
PacI 2 4772, 7702
PciI 3 143, 3922, 4970
PfoI 2 2127, 2643
PmeI 1 3747
PmlI 1 1033
PpuMI 2 2110, 3276
PsiI 3 1374, 4014, 7312
PspOMI 2 2582, 2888
PstI 5 1747, 1774, 2204, 2937, 6104
PvuI 1 987
PvuII 7 1066, 1929, 2837, 2864, 2940
6323, 6683
RsaI 10 193, 556, 581, 636, 669
720, 877, 941, 3217, 6884
RsrII 1 7094
SacI 2 1604, 3607
SacII 2 1787, 3441
SalI 1 946
SapI 3 4847, 6928, 7138
SexAI 1 3632
SfcI 8 1747, 1774, 2204, 2937, 5235
5426, 6104, 7660
SfoI 3 3481, 6330, 6578
SgrAI 1 186
SmaI 3 1903, 2083, 4187
SmlI 7 959, 3346, 3736, 5076, 5338
5615, 6970
SnaBI 1 676
SphI 3 1624, 3100, 6980
StyI 9 698, 1632, 2317, 3417, 3453
3471, 4297, 6388, 7011
TatI 5 192, 555, 635, 668, 719
TfiI 7 1893, 2434, 3542, 4149, 4945
7064, 7198
TspGWI 4 154, 3716, 4027, 4589
Tth111I 2 1721, 6694
Van91I 3 1013, 1032, 2128
XbaI 1 1240
XcmI 3 2010, 3601, 6388
XhoI 1 959
XmaI 3 1903, 2083, 4187
XmnI 1 7692
ZraI 4 459, 512, 595, 781
Enzymes that do NOT cut molecule:
AflII AgeI AleI AscI AsiSI
BbvCI BlpI BmtI BsiWI BstBI
EcoNI FseI FspAI NheI NruI
PshAI SanDI SbfI ScaI SfiI
SpeI SrfI SspI StuI SwaI