我的性奴的肉玩具1一17心奴,天堂a免费视频在线观看,久久久久久欧美精品无码,26uuu国产精品色噜噜

產(chǎn)品資料

pUMVC

如果您對(duì)該產(chǎn)品感興趣的話,可以
產(chǎn)品名稱: pUMVC
產(chǎn)品型號(hào):
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無相關(guān)文檔

簡單介紹

pUMVC的各批次質(zhì)粒菌株發(fā)貨前均經(jīng)過嚴(yán)格的多重驗(yàn)證,如存在質(zhì)量問題,請(qǐng)?jiān)谑盏疆a(chǎn)品的三個(gè)月內(nèi)通知我司。收到pUMVC后請(qǐng)短暫離心,取2μl轉(zhuǎn)化至對(duì)應(yīng)感受態(tài)中,挑取單克隆重新提取質(zhì)粒后使用。


pUMVC  的詳細(xì)介紹

pUMVC載體基本信息

試劑名稱: pUMVC
質(zhì)粒類型: 哺乳動(dòng)物載體;逆病毒包裝載體;雙質(zhì)粒包裝系統(tǒng)
高拷貝/低拷貝: 低拷貝
克隆方法: 限制性內(nèi)切酶,多克隆位點(diǎn)
啟動(dòng)子: CMV
載體大小: 9287 bp
5' 測(cè)序引物及序列: CMV fwd 5’CGCAAATGGGCGGTAGGCGTG 3’
3' 測(cè)序引物及序列: --
載體標(biāo)簽:
載體抗性: Kanamycin.html' target='_blank'>卡那霉素
篩選標(biāo)記:
克隆菌株: DH5α等
宿主細(xì)胞(系): 包裝細(xì)胞系如293T
備注: 逆病毒包裝載體pUMVC需要與信封質(zhì)粒 pCMV-VSV-G  或者pCMV-VSVG 共同使用,使用方法見下文。
產(chǎn)品目錄號(hào): #8449
穩(wěn)定性: 瞬表達(dá)
組成型/誘導(dǎo)型: 組成型
病毒/非病毒: 非病毒

pUMVC載體質(zhì)粒圖譜和多克隆位點(diǎn)信息

pUMVC載體圖譜

pUMVC載體簡介

雙質(zhì)粒系統(tǒng)逆病毒包裝質(zhì)粒pUMVC使用方法 Day 1 1.      Plate 5 x 105 293T cells in 6 cm2 dishes containing 5 mL of media. (This can be scaled up if desired) Day 2 1.      Set up (use polypropylene tubes for this; polystyrene tubes DO NOT work!): 1 μg retroviral DNA encoding gene X
           1 μg packaging plasmid Mix
                the packaging plasmid (pUMVC3) at a 8:1 ratio with the envelope plasmid (pCVM-VSV-G)-a total of 1μg
           DME without serum to 94μL total
           6 μL Fugene Mix and wait 15 to 30 minutes at room temperature

       Add to 293T cells without touching the sides of the dish (DO NOT CHANGE MEDIA)

       If you are using amphotropic virus then move immediately to BL2+ in a secondary container, which has an absorbent material. 

       (This does not mean a couple of hours; it means Immediately!).  The rest of this protocol is the same for all viruses---the BL2+ safety practices are in place if you are using amphotropic viruses Day 3 1.      Change the media to whatever media you wish to use when infecting target cells. 293T cells are easily detached so remember not to put the media directly onto to cells, but rather “run” it down the side of the dish. Remember that you will get the highest titer virus when your cells are “happy.”

2.      Plate out your target cells Day 4 1.      Remove the medium from the 293T cells and use a 0.45 u syringe filter to remove any 293T cells. DO NOT use the 0.2 u filter, as it is likely to shear the envelope from your virus
making it noninfectious

        Note: After filtering, the filter should be removed and placed in the biohazard bag in the hood and the syringe rinsed with bleach and decontaminated for a minimum of 20 min. It is useful to place a plastic beaker with bleach in the hood in advance

2.      Add 8 to 10 μg/ml of polybrene (Hexadimethrine bromide) or protamine sulfate to the target cells

3.      Carry out infection for 1 to 4 hours. Remove virus and replace with fresh media

        Note: If you wish to do a second infection the following day, it is important to put fresh media on the cells and not let the virus remain on the cells overnight. The media contains huge amounts of envelope, both associated and unassociated with viral particles, which will bind all the cell surface receptors required for virus adsorption, resulting in their down-regulation. Hence, if you don’t change the media after the initial infection, very few receptors will be available for the next round of infection. In addition, very few cells tolerate the presence of high levels of the VSV-G envelope for extended periods of time (i.e. a lot of your cells may die) Day 5 + 1.      Allow the cells to recover and begin to express the virus-encoded genes. The cells usually require 48 hours for this to occur

2.      Add drug if you are scoring for the presence of a vector that carries a drug resistance marker. Prior to this step it is advisable that you titrate the drug to be used for selection in order to know precisely how much to add. In addition, it is necessary to bring an extra plate of uninfected cells (often referred to as “canaries”) which will function as a positive control in the kill assay.  Add drug to both plates. When your canaries are dead, you can remove the drug. 

pUMVC載體序列

hz-2247R AHA3  AHA3抗體(擬南芥)
hz-4171R Angiomotin  血管抑素結(jié)合蛋白抗體
hz-4193R HIP4/CBS  絲氨酸羧甲半胱氨酸合成酶抗體
hz-1004R ACE2  血管緊張素轉(zhuǎn)換酶2抗體
hz-0203R ACEI  血管緊張素1轉(zhuǎn)換酶抑制劑抗體
hz-2745R Acetyl CoA Carboxylase  乙酰輔酶A羧化酶抗體
hz-3036R Phospho-Acetyl CoA Carboxylase(Ser79)  磷酸化乙酰輔酶A羧化酶抗體
hz-1962R ACD  腎上腺皮質(zhì)發(fā)育異常蛋白抗體
hz-2511R ACHE/Acetylcholinesterase  乙酰膽堿酶抗體
hz-0120R Acinus  Acinus抗體
hz-3001R phospho-Acinus(Ser1180)  磷酸化腺泡Acinus蛋白抗體
hz-1227R Ack1  醋酸激酶1抗體
hz-3038R Phospho-Ack1(Tyr859/860)  磷酸化Ack1抗體
hz-3045R Phospho-Ack1(Tyr284)  磷酸化Ack1抗體
hz-3046R Phospho-Ack1(Tyr326)  磷酸化Ack1抗體
hz-5022R ACSL1  長鏈脂肪酸輔酶A連接酶1/2抗體
hz-0443R ACTH (1-39)  促腎上腺皮質(zhì)**(1-39)抗體
hz-3678R Phospho-MKP1 (Ser318)  磷酸化絲裂原活化蛋白激酶磷酸酶1抗體
hz-0442R ACTH (18-39)  促腎上腺皮質(zhì)**ACTH(18-39)抗體
hz-0004R ACTH (7-23)  促腎上腺皮質(zhì)**ACTH (7-23)抗體

滬公網(wǎng)安備 31011702004356號(hào)

乳源| 盖州市| 高雄市| 罗源县| 阿合奇县| 巴彦县| 昭苏县| 南昌市| 宁河县| 营山县| 滨州市| 永昌县| 新建县| 墨江| 兴文县| 神木县| 富川| 尼勒克县| 孟连| 伊金霍洛旗| 区。| 桐柏县| 新丰县| 江达县| 理塘县| 泌阳县| 萍乡市| 宝应县| 涿鹿县| 广南县| 云梦县| 馆陶县| 祁阳县| 大庆市| 闻喜县| 淮滨县| 新平| 安远县| 惠东县| 靖远县| 青冈县|