pBAD-DEST49載體基本信息
載體名稱(chēng): | pBAD-DEST49 |
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質(zhì)粒類(lèi)型: | 大腸桿菌表達(dá)載體;Gateway載體 |
高拷貝/低拷貝: | 低拷貝 |
克隆方法: | Gateway |
啟動(dòng)子: | araBAD |
載體大小: | 6160 bp |
5' 測(cè)序引物及序列: | pBAD Forward |
3' 測(cè)序引物及序列: | -- |
載體標(biāo)簽: | V5 Epitope Tag, Thioredoxin(N-端), His Tag (C-端) |
載體抗性: | 氨芐青霉素(Ampicillin)、氯霉素(僅空載體) |
克隆菌株: | DB3.1(空載體復(fù)制);TOP10(表達(dá)載體復(fù)制) |
表達(dá)菌株: | BL21系列 |
備注: | 含EK (Enterokinase)切割位點(diǎn),切除N-端的HP-thioredoxin標(biāo)簽。 |
產(chǎn)品目錄號(hào): | 12283-016 |
穩(wěn)定性: | 穩(wěn)表達(dá) |
組成型/誘導(dǎo)型: | 誘導(dǎo)型(阿拉伯糖) |
病毒/非病毒: | 非病毒 |
The pBAD-DEST49 Gateway destination vector is designed for rapid cloning with a Gateway entry clone using lambda phage site-specific recombination and subsequent high-level, tightly regulated expression in E. coli. The pBAD-DEST49 vector features:
The araBAD promoter for tightly regulated expression in E. coli
HP-thioredoxin fusion for improved protein yield and solubility
Enterokinase cleavage site for efficient cleavage of the N-terminal fusion with EKMax
C-terminal V5 and 6xHis tags for efficient detection and purification of fusion proteins
R sites for efficient recombination with any attL-flanked Gateway entry vector