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產(chǎn)品名稱: Mouse Raf1 Antibody
產(chǎn)品型號(hào):
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無相關(guān)文檔
簡(jiǎn)單介紹
Mouse Raf1 Antibody迅速增長(zhǎng)的產(chǎn)品線包括一抗、二抗、**測(cè)定及細(xì)胞檢測(cè)試劑盒、蛋白/多肽、激動(dòng)劑/拮抗劑/活化劑/抑制劑及裂解物等。Mouse Raf1 Antibody嚴(yán)格的質(zhì)量控制和驗(yàn)證實(shí)驗(yàn)包括各種不同的應(yīng)用：WB、細(xì)胞**熒光、**組化、流式細(xì)胞術(shù)、ELISA、CHIP、IP和peptide array。
Mouse Raf1 Antibody
的詳細(xì)介紹
Mouse Raf1 AntibodyProduct Profile
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Product Name
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Mouse Raf1 Antibody
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Antibody Type
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Primary Antibodies
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Product description
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Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3 (By similarity).1) Niault, T.S., et al. Carcinogenesis 31(7):1165-1174(2010)
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Immunogen
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This Mouse Raf1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-113 amino acids from the N-terminal region of mouse Raf1.
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Mouse Raf1 AntibodyKey Feature
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Clonality
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Polyclonal
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Isotype
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Ig
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Host Species
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Rabbit
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Tested Applications
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FACSIHC-PWB
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For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50:
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Species Reactivity
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HumanMouse
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Concentration
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1mg/ml
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Purification
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Affinity purified
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Mouse Raf1 AntibodyTarget Information
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Gene Symbol
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Raf1
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Alternative Names
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RAF proto-oncogene serine/threonine-protein kinase
Proto-oncogene c-RAF
cRaf
Raf-1
Raf1
Craf
More
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Molecular Weight(MW)
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73 kDa
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Sequence Similarities
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Predicted species reactivity based on immunogen sequence: Rat
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Mouse Raf1 AntibodyDatabase Links
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Entrez Gene
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110157
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Protein Accession
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Q99N57
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Mouse Raf1 AntibodyApplication
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Application
Mouse Raf1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Application
Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
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Application
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).
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Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).
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Application Notes
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For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50:
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Additional Information
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Form
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Liquid
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Storage Instructions
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Store at 4?C for three months and -20?C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Storage Buffer
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Supplied in PBS with 0.09% (W/V) sodium azide.
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