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產(chǎn)品名稱: HSP27 Antibody
產(chǎn)品型號(hào):
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無(wú)相關(guān)文檔
簡(jiǎn)單介紹
HSP27 Antibody迅速增長(zhǎng)的產(chǎn)品線包括一抗、二抗、**測(cè)定及細(xì)胞檢測(cè)試劑盒、蛋白/多肽、激動(dòng)劑/拮抗劑/活化劑/抑制劑及裂解物等。HSP27 Antibody嚴(yán)格的質(zhì)量控制和驗(yàn)證實(shí)驗(yàn)包括各種不同的應(yīng)用：WB、細(xì)胞**熒光、**組化、流式細(xì)胞術(shù)、ELISA、CHIP、IP和peptide array。
HSP27 Antibody
的詳細(xì)介紹
HSP27 AntibodyProduct Profile
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Product Name
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HSP27 Antibody
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Antibody Type
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Primary Antibodies
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Product description
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In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSBP1 exhibits rapid increased phosphorylation in response to various mitogens, tumor promoters (e.g. phorbol esters) and calcium ionophores, and high levels are associated with carcinoma of the breast and with endometrial adenocarcinomas. Heat shock of HeLa cell cultures, or treatment with arsenite, phorbol ester, or tumor necrosis factor, causes a rapid phosphorylation of preexisting HSBP1, with Ser82 as the major site and Ser78 the minor site of phosphorylation. HSBP1 may exert phosphorylation-activated functions linked with growth signaling pathways in unstressed cells. A homeostatic function at this level could protect cells from adverse effects of signal transduction systems which may be activated inappropriately during stress.1) Wano, C., et al., Exp. Cell Res. 298(2):584-592 (2004).
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Immunogen
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This HSP27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-136 amino acids from the Central region of human HSP27.
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HSP27 AntibodyKey Feature
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Clonality
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Polyclonal
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Isotype
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Ig
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Host Species
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Rabbit
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Tested Applications
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IHC-PWB
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For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100:
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Species Reactivity
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Human
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Concentration
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1mg/ml
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Purification
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Unpurified
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HSP27 AntibodyTarget Information
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Gene Symbol
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HSPB1
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Alternative Names
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Heat shock protein beta-1
HspB1
28 kDa heat shock protein
Estrogen-regulated 24 kDa protein
Heat shock 27 kDa protein
HSP 27
Stress-responsive protein 27
SRP27
HSPB1
HSP27
HSP28
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Molecular Weight(MW)
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23 kDa
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Sequence Similarities
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Predicted species reactivity based on immunogen sequence: Bovine
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HSP27 AntibodyDatabase Links
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Entrez Gene
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3315
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Protein Accession
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P04792
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HSP27 AntibodyApplication
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Application
Hsp 27 Antibody IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Application
Western blot analysis of HSP27 antibody in MCF-7 cell line lysates (35ug/lane)
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Application Notes
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For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100:
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Additional Information
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Form
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Liquid
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Storage Instructions
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Store at 4?C for three months and -20?C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Storage Buffer
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Supplied in PBS with 0.09% (W/V) sodium azide.
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