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產(chǎn)品資料

KIT Antibody

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產(chǎn)品名稱: KIT Antibody
產(chǎn)品型號:
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無相關(guān)文檔

簡單介紹

KIT Antibody迅速增長的產(chǎn)品線包括一抗、二抗、**測定及細胞檢測試劑盒、蛋白/多肽、激動劑/拮抗劑/活化劑/抑制劑及裂解物等。KIT Antibody嚴格的質(zhì)量控制和驗證實驗包括各種不同的應用:WB、細胞**熒光、**組化、流式細胞術(shù)、ELISA、CHIP、IP和peptide array。


KIT Antibody  的詳細介紹

KIT AntibodyProduct Profile

Product Name KIT Antibody
Antibody Type Primary Antibodies
Product description
					
KIT is the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism.1) Wardelmann, E., et al., Int. J. Cancer 106(6):887-895 (2003). Lennartsson, J., et al., Exp. Cell Res. 288(1):110-118 (2003). Sakuma, Y., et al., Cancer Sci 94(6):486-491 (2003). Araki, K., et al., Lung Cancer 40(2):173-180 (2003). Voytyuk, O., et al., J. Biol. Chem. 278(11):9159-9166 (2003).
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Immunogen
This KIT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-135 amino acids from the N-terminal region of human KIT.

KIT AntibodyKey Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

FACSIHC-PWB

For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50:
Species Reactivity

HumanMouse

Concentration 1mg/ml
Purification Unpurified

KIT AntibodyTarget Information

Gene Symbol KIT
Alternative Names
Mast/stem cell growth factor receptor Kit
SCFR
Piebald trait protein
PBT
Proto-oncogene c-Kit
Tyrosine-protein kinase Kit
p145 c-kit
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
CD117
KIT
SCFR
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Molecular Weight(MW) 110 kDa

KIT AntibodyDatabase Links

Entrez Gene 3815
Protein Accession P10721

KIT AntibodyApplication

  • Application

    Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Application

    KIT Antibody (N-term D121) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use o for immunohistochemistry.

  • Application

    Western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the KIT antibody detected the KIT protein (arrow).

  • Application

    Flow cytometric analysis of NCI-H460 cells usin(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Application

    Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.

Application Notes
For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50:

Additional Information

Form Liquid
Storage Instructions Store at 4?C for three months and -20?C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.

滬公網(wǎng)安備 31011702004356號

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