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產(chǎn)品名稱(chēng): IGF1R Antibody
產(chǎn)品型號(hào):
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無(wú)相關(guān)文檔
簡(jiǎn)單介紹
IGF1R Antibody迅速增長(zhǎng)的產(chǎn)品線(xiàn)包括一抗、二抗、**測(cè)定及細(xì)胞檢測(cè)試劑盒、蛋白/多肽、激動(dòng)劑/拮抗劑/活化劑/抑制劑及裂解物等。IGF1R Antibody嚴(yán)格的質(zhì)量控制和驗(yàn)證實(shí)驗(yàn)包括各種不同的應(yīng)用：WB、細(xì)胞**熒光、**組化、流式細(xì)胞術(shù)、ELISA、CHIP、IP和peptide array。
IGF1R Antibody
的詳細(xì)介紹
IGF1R AntibodyProduct Profile
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Product Name
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IGF1R Antibody
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Antibody Type
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Primary Antibodies
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Product description
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The IGF1R receptor binds insulin-like growth factor with a high affinity and plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. The protein possess tyrosine kinase activity.1) Song, R.X., et al., Proc. Natl. Acad. Sci. U.S.A. 101(7):2076-2081 (2004).
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Immunogen
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This IGF1R antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-77 amino acids from the N-terminal region of human IGF1R.
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IGF1R AntibodyKey Feature
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Clonality
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Polyclonal
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Isotype
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Ig
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Host Species
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Rabbit
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Tested Applications
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FACSIFIHC-PWB
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For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50:
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Species Reactivity
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Human
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Concentration
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1mg/ml
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Purification
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Unpurified
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IGF1R AntibodyTarget Information
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Gene Symbol
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IGF1R
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Alternative Names
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Insulin-like growth factor 1 receptor
Insulin-like growth factor I receptor
IGF-I receptor
CD221
Insulin-like growth factor 1 receptor alpha chain
Insulin-like growth factor 1 receptor beta chain
IGF1R
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Molecular Weight(MW)
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155 kDa
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Sequence Similarities
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Predicted species reactivity based on immunogen sequence: Mouse
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IGF1R AntibodyDatabase Links
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Entrez Gene
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3480
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Protein Accession
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P08069
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IGF1R AntibodyApplication
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Application
Confocal immunofluorescent analysis of IGF1R Antibody (N-term K66) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Application
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Application
IGF1R Antibody (N-term K66) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IGF1R Antibody (N-term K66) for immunohistochemistry.
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Application
Western blot analysis of IGF1R Antibody (N-term K66) in WiDr cell line lysates (35ug/lane)
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Application Notes
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For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50:
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Additional Information
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Form
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Liquid
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Storage Instructions
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Store at 4?C for three months and -20?C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Storage Buffer
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Supplied in PBS with 0.09% (W/V) sodium azide.
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