Tyrosine Hydroxylase (phospho Ser19) Antibody
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產(chǎn)品名稱: Tyrosine Hydroxylase (phospho Ser19) Antibody
產(chǎn)品型號:
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
Tyrosine Hydroxylase (phospho Ser19) Antibody迅速增長的產(chǎn)品線包括一抗、二抗、**測定及細(xì)胞檢測試劑盒、蛋白/多肽、激動劑/拮抗劑/活化劑/抑制劑及裂解物等。Tyrosine Hydroxylase (phospho Ser19) Antibody嚴(yán)格的質(zhì)量控制和驗證實驗包括各種不同的應(yīng)用：WB、細(xì)胞**熒光、**組化、流式細(xì)胞術(shù)、ELISA、CHIP、IP和peptide array。
Tyrosine Hydroxylase (phospho Ser19) Antibody
的詳細(xì)介紹
Tyrosine Hydroxylase (phospho Ser19) AntibodyProduct Profile
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Product Name
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Tyrosine Hydroxylase (phospho Ser19) Antibody
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Antibody Type
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Primary Antibodies
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Product description
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Tyrosine Hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons. We raised this polyclonal antibody against a peptide representing the sequence around Ser19 in rat TH purified. This antibody is suitable for most immunochemical applications in a variety of mammalian and some non-mammalian species.1) Witkovsky, P., Gabriel, R., Haycock, J. W., and Meller, E., "Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina," J. Chem. Neuroanat., 19 (2000) 105 - 116.
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Immunogen
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TH (Ser19) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser19 of rat tyrosine hydroxylase (TH).
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Tyrosine Hydroxylase (phospho Ser19) AntibodyKey Feature
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Clonality
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Polyclonal
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Host Species
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Rabbit
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Tested Applications
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IFIHCWB
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Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Initial recommended range of dilutions: 1:1000 to 1:2000. Applications include Dot Blots (DB), Immunohistochemistry (IHC), Immunofluorescence (IF) and Western Blot (WB). When internally tested under ideal conditions the working dilutions were 1:1000 for IF, IHC, DB and WB.:
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Species Reactivity
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Rat
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Concentration
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1mg/ml
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Purification
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Affinity purified
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Tyrosine Hydroxylase (phospho Ser19) AntibodyTarget Information
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Gene Symbol
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Th
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Alternative Names
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The
Tyrosine 3-hydroxylase
TH
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Molecular Weight(MW)
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60
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Tissue Specificity
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Tyrosine Hydroxylase antibody is specific for the 60k Tyrosine Hydroxylase phosphorylated at Ser19 in PC-12 cells.
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Tyrosine Hydroxylase (phospho Ser19) AntibodyDatabase Links
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Entrez Gene
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25085
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Protein Accession
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P04177
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Tyrosine Hydroxylase (phospho Ser19) AntibodyApplication
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Application
Tyrosine Hydroxylase (Phospho-Ser19) Antibody
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Application Notes
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Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Initial recommended range of dilutions: 1:1000 to 1:2000. Applications include Dot Blots (DB), Immunohistochemistry (IHC), Immunofluorescence (IF) and Western Blot (WB). When internally tested under ideal conditions the working dilutions were 1:1000 for IF, IHC, DB and WB.:
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Additional Information
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Form
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Liquid
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Storage Instructions
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For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles.
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Storage Buffer
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100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
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