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產(chǎn)品名稱: Vimentin Antibody
產(chǎn)品型號:
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
Vimentin Antibody迅速增長的產(chǎn)品線包括一抗、二抗、**測定及細(xì)胞檢測試劑盒、蛋白/多肽、激動劑/拮抗劑/活化劑/抑制劑及裂解物等。Vimentin Antibody嚴(yán)格的質(zhì)量控制和驗證實驗包括各種不同的應(yīng)用：WB、細(xì)胞**熒光、**組化、流式細(xì)胞術(shù)、ELISA、CHIP、IP和peptide array。
Vimentin Antibody
的詳細(xì)介紹
Vimentin AntibodyProduct Profile
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Product Name
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Vimentin Antibody
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Antibody Type
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Primary Antibodies
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Product description
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Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.1) References for protein:
More
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Immunogen
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This Vimentin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 430-457 amino acids from the C-terminal region of human Vimentin.
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Vimentin AntibodyKey Feature
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Clonality
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Polyclonal
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Isotype
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Ig
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Host Species
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Rabbit
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Tested Applications
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FACSIFIHC-PWB
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For WB starting dilution is: 1:1000 For IF starting dilution is: 1:100 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50:
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Species Reactivity
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Human
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Concentration
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1mg/ml
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Purification
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Unpurified
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Vimentin AntibodyTarget Information
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Gene Symbol
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VIM
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Alternative Names
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Vimentin
VIM
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Molecular Weight(MW)
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54 kDa
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Sequence Similarities
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Predicted species reactivity based on immunogen sequence: Xenopus
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Vimentin AntibodyDatabase Links
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Entrez Gene
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7431
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Protein Accession
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P08670
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Vimentin AntibodyApplication
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Application
Western Blot at 1:1000 dilution + U-2OS whole cell lysate Lysates/proteins at 20 ug per lane.
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Application
Fluorescent confocal image of SY5Y cells stained with Vimentin antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with Antibody Vimentin primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstaine
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Application
Western blot analysis of Vimentin antibody in NCI-H460 cell line lysates (35ug/lane).
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Application
Vimentin Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Application Notes
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For WB starting dilution is: 1:1000 For IF starting dilution is: 1:100 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50:
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Additional Information
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Form
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Liquid
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Storage Instructions
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Store at 4?C for three months and -20?C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Storage Buffer
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Supplied in PBS with 0.09% (W/V) sodium azide.
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