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產(chǎn)品名稱: Mouse Map2k1 Antibody
產(chǎn)品型號:
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
Mouse Map2k1 Antibody迅速增長的產(chǎn)品線包括一抗、二抗、**測定及細(xì)胞檢測試劑盒、蛋白/多肽、激動劑/拮抗劑/活化劑/抑制劑及裂解物等。Mouse Map2k1 Antibody嚴(yán)格的質(zhì)量控制和驗證實驗包括各種不同的應(yīng)用：WB、細(xì)胞**熒光、**組化、流式細(xì)胞術(shù)、ELISA、CHIP、IP和peptide array。
Mouse Map2k1 Antibody
的詳細(xì)介紹
Mouse Map2k1 AntibodyProduct Profile
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Product Name
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Mouse Map2k1 Antibody
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Antibody Type
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Primary Antibodies
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Product description
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Map2k1 catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.1) Chandrakesan, P., et al. J. Biol. Chem. 285(43):33485-33498(2010)
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Immunogen
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This Mouse Map2k1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 269-296 amino acids from the C-terminal region of mouse Map2k1.
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Mouse Map2k1 AntibodyKey Feature
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Clonality
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Polyclonal
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Isotype
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Ig
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Host Species
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Rabbit
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Tested Applications
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IHC-PWB
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For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50:
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Species Reactivity
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HumanMouse
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Concentration
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1mg/ml
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Purification
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Affinity purified
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Mouse Map2k1 AntibodyTarget Information
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Gene Symbol
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Map2k1
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Alternative Names
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Dual specificity mitogen-activated protein kinase kinase 1
MAP kinase kinase 1
MAPKK 1
ERK activator kinase 1
MAPK/ERK kinase 1
MEK 1
Map2k1
Mek1
Prkmk1
More
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Molecular Weight(MW)
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43 kDa
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Sequence Similarities
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Predicted species reactivity based on immunogen sequence: Hamster
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Mouse Map2k1 AntibodyDatabase Links
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Entrez Gene
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26395
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Protein Accession
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P31938
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Mouse Map2k1 AntibodyApplication
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Application
Mouse Map2k1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Application
Western blot analysis in mouse cerebellum tissue lysates (35ug/lane).
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Application
Western blot analysis of Map2k1 using rabbit polyclonal Mouse Map2k1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Map2k1 gene.
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Application
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
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Application Notes
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For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50:
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Additional Information
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Form
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Liquid
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Storage Instructions
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Store at 4?C for three months and -20?C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Storage Buffer
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Supplied in PBS with 0.09% (W/V) sodium azide.
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